A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis

Tetsuro Kuga; Motoo Yamasaki; Susumu Sekine; Masanori Fukui; Yoshiharu Yokoo; Seiga Itoh; Mitsuaki Yoshida; Toshio Hattori; Kiyoshi Takatsuki

A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis

Tetsuro Kuga, Motoo Yamasaki, Susumu Sekine, Masanori Fukui, Yoshiharu Yokoo, Seiga Itoh, Mitsuaki Yoshida, Toshio Hattori, Kiyoshi Takatsuki

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.

Original language	English
Pages (from-to)	1168-1173
Number of pages	6
Journal	Japanese Journal of Cancer Research
Volume	79
Issue number	11
Publication status	Published - 1988 Nov

Keywords

Antibodies
Diagnosis
HTLV-I
Hybrid antigen

ASJC Scopus subject areas

Oncology
Cancer Research

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A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis. / Kuga, Tetsuro; Yamasaki, Motoo; Sekine, Susumu; Fukui, Masanori; Yokoo, Yoshiharu; Itoh, Seiga; Yoshida, Mitsuaki; Hattori, Toshio; Takatsuki, Kiyoshi.

In: Japanese Journal of Cancer Research, Vol. 79, No. 11, 11.1988, p. 1168-1173.

Research output: Contribution to journal › Article › peer-review

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