A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis

Tetsuro Kuga, Motoo Yamasaki, Susumu Sekine, Masanori Fukui, Yoshiharu Yokoo, Seiga Itoh, Mitsuaki Yoshida, Toshio Hattori, Kiyoshi Takatsuki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.

Original languageEnglish
Pages (from-to)1168-1173
Number of pages6
JournalJapanese Journal of Cancer Research
Volume79
Issue number11
Publication statusPublished - 1988 Nov

Keywords

  • Antibodies
  • Diagnosis
  • HTLV-I
  • Hybrid antigen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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