Abstract
A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.
Original language | English |
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Pages (from-to) | 1168-1173 |
Number of pages | 6 |
Journal | Japanese Journal of Cancer Research |
Volume | 79 |
Issue number | 11 |
Publication status | Published - 1988 Nov |
Keywords
- Antibodies
- Diagnosis
- HTLV-I
- Hybrid antigen
ASJC Scopus subject areas
- Oncology
- Cancer Research