TY - JOUR
T1 - A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells
AU - Kashima, Isao
AU - Takahashi, Masaki
AU - Hashimoto, Yoshifumi
AU - Sakota, Eri
AU - Nakamura, Yoshikazu
AU - Inada, Toshifumu
PY - 2014/11
Y1 - 2014/11
N2 - When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF1) and Ltn1 (an E3 ubiquitin ligase) play crucial roles in NSD and RQC, respectively, by triggering ribosome rescue at the 3' end of nonstop mRNAs and proteasome-dependent polypeptide degradation. Here we confirmed the essential role of Ltn1 in RQC for nonstop products in Drosophila cells, and further uncovered a functional role of ABCE1, a eukaryotic ribosome recycling factor, in NSD in Drosophila cells.
AB - When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF1) and Ltn1 (an E3 ubiquitin ligase) play crucial roles in NSD and RQC, respectively, by triggering ribosome rescue at the 3' end of nonstop mRNAs and proteasome-dependent polypeptide degradation. Here we confirmed the essential role of Ltn1 in RQC for nonstop products in Drosophila cells, and further uncovered a functional role of ABCE1, a eukaryotic ribosome recycling factor, in NSD in Drosophila cells.
KW - ABCE1
KW - Nonstop decay (NSD)
KW - Protein surveillance
KW - Translation termination
KW - mRNA surveillance
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UR - http://www.scopus.com/inward/citedby.url?scp=84908030206&partnerID=8YFLogxK
U2 - 10.1016/j.biochi.2014.08.001
DO - 10.1016/j.biochi.2014.08.001
M3 - Article
C2 - 25128630
AN - SCOPUS:84908030206
VL - 106
SP - 10
EP - 16
JO - Biochimie
JF - Biochimie
SN - 0300-9084
ER -