A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells

Isao Kashima, Masaki Takahashi, Yoshifumi Hashimoto, Eri Sakota, Yoshikazu Nakamura, Toshifumu Inada

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF1) and Ltn1 (an E3 ubiquitin ligase) play crucial roles in NSD and RQC, respectively, by triggering ribosome rescue at the 3' end of nonstop mRNAs and proteasome-dependent polypeptide degradation. Here we confirmed the essential role of Ltn1 in RQC for nonstop products in Drosophila cells, and further uncovered a functional role of ABCE1, a eukaryotic ribosome recycling factor, in NSD in Drosophila cells.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalBiochimie
Volume106
DOIs
Publication statusPublished - 2014 Nov

Keywords

  • ABCE1
  • Nonstop decay (NSD)
  • Protein surveillance
  • Translation termination
  • mRNA surveillance

ASJC Scopus subject areas

  • Biochemistry

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