A full validated hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin in human plasma ultrafiltrates

Hajime Ito, Hiroaki Yamaguchi, Asuka Fujikawa, Nobuaki Tanaka, Ayako Furugen, Kazuaki Miyamori, Natsuko Takahashi, Jiro Ogura, Masaki Kobayashi, Takehiro Yamada, Nariyasu Mano, Ken Iseki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Oxaliplatin is a platinum agent that is used for treatment of colorectal cancer. A sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the quantification of oxaliplatin was developed. Human plasma ultrafiltrates were precipitated by acetonitrile containing carboplatin as an internal standard and further diluted with acetonitrile. Chromatographic separation of oxaliplatin and the internal standard was achieved with a column modified with phosphorylcholine and an isocratic mobile phase (acetonitrile/water/acetic acid = 90:10:0.1, v/v/v) at the flow rate of 0.2. mL/min. The lower limit of quantification for oxaliplatin was 25. ng/mL. The linearity range of the method was from 25 to 5000. ng/mL. The intra-day precision and inter-day precision (RSD) ranged from 0.8 to 6.1%, and the accuracy (RE) was within ±4.5%. The extraction recoveries from human plasma ultrafiltrates were 83.6-91.6%, and ion suppression caused by matrix components was 86.7-88.5% at three different levels, respectively. This method was applied to a clinical pharmacokinetic study of oxaliplatin in a cancer patient. The maximum concentration of colorectal cancer patient administered oxaliplatin was 1650 ng/mL.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume71
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Hydrophilic interaction liquid chromatography-tandem mass spectrometry
  • Oxaliplatin
  • Plasma ultrafiltrates

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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