A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC)

Yoshiyuki Tanaka, Satoshi Fujii, Hidekazu Hiroaki, Takeshi Sakata, Toshiki Tanaka, Seiichi Uesugi, Ken Ichi Tomita, Yoshimasa Kyogoku

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Here we demonstrate the presence of the A'-RNA conformation using the single crystal structure of a tridecamer: r(UGAGCUUCGGCUC). The average A'-RNA conformation deduced from X-ray fiber diffraction data had only been available previously, but now the presence of the A'-RNA conformation has been found in a single crystal structure for the first time. Statistical analysis showed that the A'-RNA conformation is distinguishable from the A-RNA conformation in a plot of the major groove width against the base pair inclination angle. The major groove of the A'-RNA conformation is wide enough to accommodate a protein or peptide while that of the A-RNA conformation is too narrow to do so. The presence of the A'-RNA conformation is significant for protein-RNA interaction.

Original languageEnglish
Pages (from-to)949-955
Number of pages7
JournalNucleic acids research
Volume27
Issue number4
DOIs
Publication statusPublished - 1999 Feb 15

ASJC Scopus subject areas

  • Genetics

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    Tanaka, Y., Fujii, S., Hiroaki, H., Sakata, T., Tanaka, T., Uesugi, S., Tomita, K. I., & Kyogoku, Y. (1999). A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC). Nucleic acids research, 27(4), 949-955. https://doi.org/10.1093/nar/27.4.949