A fine physical map of Arabidopsis thaliana chromosome 5: Construction of a sequence-ready contig map

Hirokazu Kotani, Shusei Sato, Masanobu Fukami, Tsutomu Hosouchi, Naomi Nakazaki, Satomi Okumura, Tsuyuko Wada, You Guang Liu, Daisuke Shibata, Satoshi Tabata

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A fine physical map of Arabidopsis thaliana chromosome 5 was constructed by ordering the clones from YAC, P1, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of P1, TAC and BAC clones. The entire genome of chromosome 5, except for the centromeric and telomeric regions, was covered by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region. The minimum tiling path of the chromosome was constituted by a total of 430 P1, TAC and BAC clones. The map information is available at the Web site http://www.kazusa.or.jp/arabi/.

Original languageEnglish
Pages (from-to)371-378
Number of pages8
JournalDNA Research
Volume4
Issue number6
DOIs
Publication statusPublished - 1997

Keywords

  • Arabidopsis thaliana chromosome 5
  • Contig map
  • Physical map

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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