TY - JOUR
T1 - A fine physical map of Arabidopsis thaliana chromosome 5
T2 - Construction of a sequence-ready contig map
AU - Kotani, Hirokazu
AU - Sato, Shusei
AU - Fukami, Masanobu
AU - Hosouchi, Tsutomu
AU - Nakazaki, Naomi
AU - Okumura, Satomi
AU - Wada, Tsuyuko
AU - Liu, You Guang
AU - Shibata, Daisuke
AU - Tabata, Satoshi
PY - 1997
Y1 - 1997
N2 - A fine physical map of Arabidopsis thaliana chromosome 5 was constructed by ordering the clones from YAC, P1, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of P1, TAC and BAC clones. The entire genome of chromosome 5, except for the centromeric and telomeric regions, was covered by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region. The minimum tiling path of the chromosome was constituted by a total of 430 P1, TAC and BAC clones. The map information is available at the Web site http://www.kazusa.or.jp/arabi/.
AB - A fine physical map of Arabidopsis thaliana chromosome 5 was constructed by ordering the clones from YAC, P1, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of P1, TAC and BAC clones. The entire genome of chromosome 5, except for the centromeric and telomeric regions, was covered by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region. The minimum tiling path of the chromosome was constituted by a total of 430 P1, TAC and BAC clones. The map information is available at the Web site http://www.kazusa.or.jp/arabi/.
KW - Arabidopsis thaliana chromosome 5
KW - Contig map
KW - Physical map
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U2 - 10.1093/dnares/4.6.371
DO - 10.1093/dnares/4.6.371
M3 - Article
C2 - 9501992
AN - SCOPUS:0031592968
VL - 4
SP - 371
EP - 378
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 6
ER -