A double chaperone function of the sHsp genes against heat-based environmental adversity in the soil-dwelling leaf beetles

Elizabeth Atungulu, Hiromasa Tanaka, Kosuke Fujita, Kei Ichiro Yamamoto, Mutsumi Sakata, Erika Sato, Michihiro Hara, Tetsuro Yamashita, Koichi Suzuki

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Small heat shock proteins (sHsps), which are constitutively expressed in the absence of stress during development in many organisms, have been correlated with not only enhanced thermoresistance but also the developmental, tissue-, and cell-specific induction and expression. This study demonstrates the constitutive sHsps 21 and 23 of an entomoresouce, the leaf beetle, Gastrophysa atrocyanea, display a double chaperone function in vitro. The RNAi suppression of two sHsp genes in adults resulted in decreased thermoresistance. A strong correlation was observed between the in vitro chaperone function and in vivo thermotolerance analysis, and the results support a possible double chaperone function critical for the survival of the leaf beetles against higher temperatures.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalJournal of Insect Biotechnology and Sericology
Volume75
Issue number1
Publication statusPublished - 2006 Feb 1
Externally publishedYes

Keywords

  • Adult diapause
  • Double chaperone function
  • Gastrophysa atrocyanea
  • RNAi
  • sHsps

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

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