A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan

Seinen Chow; Tadashi Imai; Minoru Ikeda; Soichiro Maki; Takakiyo Oonuki; Fumito Muto; Kenji Nohara; Chiharu Furusawa; Hiroshi Shichiri; Naoko Nigorikawa; Naoko Uragaki; Akiko Kawamura; Tatsuya Ichikawa; Kentaro Ushioda; Masahito Higuchi; Taro Tega; Koji Kodama; Masahiro Itoh; Masaki Ichimura; Koji Matsuzaki; Kei Hirasawa; Keita Tokura; Katsumi Nakahata; Sakie Kodama; Hiroshi Hakoyama; Takashi Yada; Kentaro Niwa; Satoshi Nagai; Takashi Yanagimoto; Kazuyoshi Saito; Mitsuhiro Nakaya; Tomoaki Maruyama

doi:10.2331/suisan.17-00082

A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan

Seinen Chow, Tadashi Imai, Minoru Ikeda, Soichiro Maki, Takakiyo Oonuki, Fumito Muto, Kenji Nohara, Chiharu Furusawa, Hiroshi Shichiri, Naoko Nigorikawa, Naoko Uragaki, Akiko Kawamura, Tatsuya Ichikawa, Kentaro Ushioda, Masahito Higuchi, Taro Tega, Koji Kodama, Masahiro Itoh, Masaki Ichimura, Koji MatsuzakiKei Hirasawa, Keita Tokura, Katsumi Nakahata, Sakie Kodama, Hiroshi Hakoyama, Takashi Yada, Kentaro Niwa, Satoshi Nagai, Takashi Yanagimoto, Kazuyoshi Saito, Mitsuhiro Nakaya, Tomoaki Maruyama

AGR - Field Science Center

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.

Original language	English
Pages (from-to)	674-681
Number of pages	8
Journal	Nippon Suisan Gakkaishi (Japanese Edition)
Volume	84
Issue number	4
DOIs	https://doi.org/10.2331/suisan.17-00082
Publication status	Published - 2018

Keywords

18S rDNA
PCR
Palaemon paucidens

ASJC Scopus subject areas

Aquatic Science

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Chow, S., Imai, T., Ikeda, M., Maki, S., Oonuki, T., Muto, F., Nohara, K., Furusawa, C., Shichiri, H., Nigorikawa, N., Uragaki, N., Kawamura, A., Ichikawa, T., Ushioda, K., Higuchi, M., Tega, T., Kodama, K., Itoh, M., Ichimura, M., ... Maruyama, T. (2018). A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan. Nippon Suisan Gakkaishi (Japanese Edition), 84(4), 674-681. https://doi.org/10.2331/suisan.17-00082

A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan. / Chow, Seinen; Imai, Tadashi; Ikeda, Minoru; Maki, Soichiro; Oonuki, Takakiyo; Muto, Fumito; Nohara, Kenji; Furusawa, Chiharu; Shichiri, Hiroshi; Nigorikawa, Naoko; Uragaki, Naoko; Kawamura, Akiko; Ichikawa, Tatsuya; Ushioda, Kentaro; Higuchi, Masahito; Tega, Taro; Kodama, Koji; Itoh, Masahiro; Ichimura, Masaki; Matsuzaki, Koji; Hirasawa, Kei; Tokura, Keita; Nakahata, Katsumi; Kodama, Sakie; Hakoyama, Hiroshi; Yada, Takashi; Niwa, Kentaro; Nagai, Satoshi; Yanagimoto, Takashi; Saito, Kazuyoshi; Nakaya, Mitsuhiro; Maruyama, Tomoaki.

In: Nippon Suisan Gakkaishi (Japanese Edition), Vol. 84, No. 4, 2018, p. 674-681.

Research output: Contribution to journal › Article › peer-review

Chow, S, Imai, T, Ikeda, M, Maki, S, Oonuki, T, Muto, F, Nohara, K, Furusawa, C, Shichiri, H, Nigorikawa, N, Uragaki, N, Kawamura, A, Ichikawa, T, Ushioda, K, Higuchi, M, Tega, T, Kodama, K, Itoh, M, Ichimura, M, Matsuzaki, K, Hirasawa, K, Tokura, K, Nakahata, K, Kodama, S, Hakoyama, H, Yada, T, Niwa, K, Nagai, S, Yanagimoto, T, Saito, K, Nakaya, M & Maruyama, T 2018, 'A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan', Nippon Suisan Gakkaishi (Japanese Edition), vol. 84, no. 4, pp. 674-681. https://doi.org/10.2331/suisan.17-00082

Chow, Seinen ; Imai, Tadashi ; Ikeda, Minoru ; Maki, Soichiro ; Oonuki, Takakiyo ; Muto, Fumito ; Nohara, Kenji ; Furusawa, Chiharu ; Shichiri, Hiroshi ; Nigorikawa, Naoko ; Uragaki, Naoko ; Kawamura, Akiko ; Ichikawa, Tatsuya ; Ushioda, Kentaro ; Higuchi, Masahito ; Tega, Taro ; Kodama, Koji ; Itoh, Masahiro ; Ichimura, Masaki ; Matsuzaki, Koji ; Hirasawa, Kei ; Tokura, Keita ; Nakahata, Katsumi ; Kodama, Sakie ; Hakoyama, Hiroshi ; Yada, Takashi ; Niwa, Kentaro ; Nagai, Satoshi ; Yanagimoto, Takashi ; Saito, Kazuyoshi ; Nakaya, Mitsuhiro ; Maruyama, Tomoaki. / A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan. In: Nippon Suisan Gakkaishi (Japanese Edition). 2018 ; Vol. 84, No. 4. pp. 674-681.

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abstract = "Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.",

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AU - Chow, Seinen

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AU - Maki, Soichiro

AU - Oonuki, Takakiyo

AU - Muto, Fumito

AU - Nohara, Kenji

AU - Furusawa, Chiharu

AU - Shichiri, Hiroshi

AU - Nigorikawa, Naoko

AU - Uragaki, Naoko

AU - Kawamura, Akiko

AU - Ichikawa, Tatsuya

AU - Ushioda, Kentaro

AU - Higuchi, Masahito

AU - Tega, Taro

AU - Kodama, Koji

AU - Itoh, Masahiro

AU - Ichimura, Masaki

AU - Matsuzaki, Koji

AU - Hirasawa, Kei

AU - Tokura, Keita

AU - Nakahata, Katsumi

AU - Kodama, Sakie

AU - Hakoyama, Hiroshi

AU - Yada, Takashi

AU - Niwa, Kentaro

AU - Nagai, Satoshi

AU - Yanagimoto, Takashi

AU - Saito, Kazuyoshi

AU - Nakaya, Mitsuhiro

AU - Maruyama, Tomoaki

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AB - Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.

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