A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan

Seinen Chow, Tadashi Imai, Minoru Ikeda, Soichiro Maki, Takakiyo Oonuki, Fumito Muto, Kenji Nohara, Chiharu Furusawa, Hiroshi Shichiri, Naoko Nigorikawa, Naoko Uragaki, Akiko Kawamura, Tatsuya Ichikawa, Kentaro Ushioda, Masahito Higuchi, Taro Tega, Koji Kodama, Masahiro Itoh, Masaki Ichimura, Koji MatsuzakiKei Hirasawa, Keita Tokura, Katsumi Nakahata, Sakie Kodama, Hiroshi Hakoyama, Takashi Yada, Kentaro Niwa, Satoshi Nagai, Takashi Yanagimoto, Kazuyoshi Saito, Mitsuhiro Nakaya, Tomoaki Maruyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.

Original languageEnglish
Pages (from-to)674-681
Number of pages8
JournalNippon Suisan Gakkaishi (Japanese Edition)
Volume84
Issue number4
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • 18S rDNA
  • PCR
  • Palaemon paucidens

ASJC Scopus subject areas

  • Aquatic Science

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    Chow, S., Imai, T., Ikeda, M., Maki, S., Oonuki, T., Muto, F., Nohara, K., Furusawa, C., Shichiri, H., Nigorikawa, N., Uragaki, N., Kawamura, A., Ichikawa, T., Ushioda, K., Higuchi, M., Tega, T., Kodama, K., Itoh, M., Ichimura, M., ... Maruyama, T. (2018). A DNA marker to discriminate two types of freshwater shrimp Palaemon paucidens and the distribution of these two types in Japan. Nippon Suisan Gakkaishi (Japanese Edition), 84(4), 674-681. https://doi.org/10.2331/suisan.17-00082