Abstract
Among the palaemonid shrimp species of the genus Palaemon known from Japan, Palaemon paucidens is the only species inhabiting freshwater areas. Two genetically distinct types (designated by A and B) were found by allozyme electrophoresis analysis three decades ago. We developed a simple multiplex-PCR assay to discriminate the two types based on 18S rDNA sequences. A total of 422 individuals collected at 152 localities in Japan were analyzed by the multiplex-PCR assay. No heterozygote was observed, indicating that these two types have been reproductively isolated. Both types were distributed throughout Japan; type A was observed in ponds, lakes and rivers, while type B was observed only in rivers.
Original language | English |
---|---|
Pages (from-to) | 674-681 |
Number of pages | 8 |
Journal | Nippon Suisan Gakkaishi (Japanese Edition) |
Volume | 84 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2018 |
Keywords
- 18S rDNA
- PCR
- Palaemon paucidens
ASJC Scopus subject areas
- Aquatic Science