A direct method for the separation and quantification of bile acid acyl glycosides by high-performance liquid chromatography with an evaporative light scattering detector

Genta Kakiyama, Atsuko Hosoda, Takashi Iida, Yasuo Fujimoto, Takaaki Goto, Nariyasu Mano, Junichi Goto, Toshio Nambara

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A direct method for the separation and quantification of a series of bile acid acyl glycosides using high-performance liquid chromatography coupled to an evaporative light scattering detector (HPLC-ELSD) is described. Complete separation of each of 15 bile acid acyl 24-α-glucosides and their 24-β-anomers and 24-β-galactosides was achieved by the stepwise gradient elution mode on a C18 column using a mixture of acetonitrile-methanol (8:2, v/v) and 1% aqueous acetic acid as the mobile phase. 24-β-Galactosides were always eluted faster than the corresponding 24-β-glucosides, which eluted after the corresponding 24-α-anomers. Calibration curves of different 24-β-galactosides were linear over a range of 0.2-40 nmol of injected amount and the detection limits (S/N > 3) were from 0.08 to 0.1 nmol. The present HPLC-ELSD method may provide an insight into the separation and quantification of the biologically interesting neutral bile acids.

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalJournal of Chromatography A
Volume1125
Issue number1
DOIs
Publication statusPublished - 2006 Aug 25

Keywords

  • Bile acid 24-galactoside
  • Bile acid 24-glucoside
  • Bile acid acyl glycoside
  • Evaporative light scattering detector
  • High-performance liquid chromatography
  • α-/β-Anomer

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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