TY - JOUR
T1 - A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage
AU - Tadakuma, Kenjiro
AU - Tanaka, Nobuyuki
AU - Haraguchi, Yuji
AU - Higashimori, Mitsuru
AU - Kaneko, Makoto
AU - Shimizu, Tatsuya
AU - Yamato, Masayuki
AU - Okano, Teruo
N1 - Funding Information:
Upon the development of the cell-sheet transfer/transplantation devise in this study, the authors referred the basic concept of SWITL, a food-handling devise, developed by Furukawakikou Co., Ltd. (Niigata, Japan). The authors are grateful to the members of Furukawakikou. This work was supported by grants from a new area of Hyper Bio Assembler for 3D Cellular Systems (BioAssembler) Project , a Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) Fellows ( 23·7758 ), the Global Center of Excellence Program , Multidisciplinary Education and Technology and Research Center for Regenerative Medicine (MERCREM) , Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science , and the High-Tech Research Center Program from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) , Japan, and JSPS through the “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program),” initiated by the Council for Science and Technology Policy (CSTP) .
PY - 2013/12
Y1 - 2013/12
N2 - In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7±1.6s) using the device compared with that of a conventional method using a pipette (430±180s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cell-cell junctions and ion channel proteins on the cell surface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine.
AB - In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7±1.6s) using the device compared with that of a conventional method using a pipette (430±180s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cell-cell junctions and ion channel proteins on the cell surface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine.
KW - Cell sheet engineering
KW - Cell viability
KW - High speed
KW - Tissue adhesive
KW - Transfer/transplantation device
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=84884202504&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884202504&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2013.08.006
DO - 10.1016/j.biomaterials.2013.08.006
M3 - Article
C2 - 23972478
AN - SCOPUS:84884202504
VL - 34
SP - 9018
EP - 9025
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 36
ER -