A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage

Kenjiro Tadakuma, Nobuyuki Tanaka, Yuji Haraguchi, Mitsuru Higashimori, Makoto Kaneko, Tatsuya Shimizu, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7±1.6s) using the device compared with that of a conventional method using a pipette (430±180s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cell-cell junctions and ion channel proteins on the cell surface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)9018-9025
Number of pages8
JournalBiomaterials
Volume34
Issue number36
DOIs
Publication statusPublished - 2013 Dec 1
Externally publishedYes

Keywords

  • Cell sheet engineering
  • Cell viability
  • High speed
  • Tissue adhesive
  • Transfer/transplantation device
  • Transplantation

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Tadakuma, K., Tanaka, N., Haraguchi, Y., Higashimori, M., Kaneko, M., Shimizu, T., Yamato, M., & Okano, T. (2013). A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage. Biomaterials, 34(36), 9018-9025. https://doi.org/10.1016/j.biomaterials.2013.08.006