A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus

Tracey Welham, Jodie Pike, Irmtraud Horst, Emmanouil Flemetakis, Panagiotis Katinakis, Takakazu Kaneko, Shusei Sato, Satoshi Tabata, Jillian Perry, Martin Parniske, Trevor L. Wang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified as encoding a nodule-enhanced isoform is the predominant isoform present in all parts of the plant. Mutants for two isoforms, LjINV1 and LjINV2, were isolated using TILLING. A premature stop codon allele of LjINV2 had no effect on enzyme activity nor did it show a visible phenotype. For LjINV1, premature stop codon and missense mutations were obtained and the phenotype of the mutants examined. Recovery of homozygous mutants was problematic, but their phenotype showed a severe reduction in growth of the root and the shoot, a change in cellular development, and impaired flowering. The cellular organization of both roots and leaves was altered; leaves were smaller and thicker with extra layers of cells and roots showed an extended and broader zone of cell division. Moreover, anthers contained no pollen. Both heterozygotes and homozygous mutants showed decreased amounts of enzyme activity in nodules and shoot tips. Shoot tips also contained up to a 9-fold increased level of sucrose. However, mutants were capable of forming functional root nodules. LjINV1 is therefore crucial to whole plant development, but is clearly not essential for nodule formation or function.

Original languageEnglish
Pages (from-to)3353-3365
Number of pages13
JournalJournal of experimental botany
Volume60
Issue number12
DOIs
Publication statusPublished - 2009 Aug
Externally publishedYes

Keywords

  • Cellular development
  • Legume
  • Lotus japonicus
  • Mutants
  • Neutral/alkaline invertase
  • Plant development
  • Sucrose metabolism
  • TILLING

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint Dive into the research topics of 'A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus'. Together they form a unique fingerprint.

  • Cite this

    Welham, T., Pike, J., Horst, I., Flemetakis, E., Katinakis, P., Kaneko, T., Sato, S., Tabata, S., Perry, J., Parniske, M., & Wang, T. L. (2009). A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus. Journal of experimental botany, 60(12), 3353-3365. https://doi.org/10.1093/jxb/erp169