A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

Takakazu Nakabayashi; Hui Ping Wang; Kazuo Tsujimoto; Seiji Miyauchi; Naoki Kamo; Nobuhiro Ohta

doi:10.1246/cl.2007.206

A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

Takakazu Nakabayashi, Hui Ping Wang, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

Original language	English
Pages (from-to)	206-207
Number of pages	2
Journal	Chemistry Letters
Volume	36
Issue number	2
DOIs	https://doi.org/10.1246/cl.2007.206
Publication status	Published - 2007 Feb 5
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.2007.206

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A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro. / Nakabayashi, Takakazu; Wang, Hui Ping; Tsujimoto, Kazuo; Miyauchi, Seiji; Kamo, Naoki; Ohta, Nobuhiro.

In: Chemistry Letters, Vol. 36, No. 2, 05.02.2007, p. 206-207.

Research output: Contribution to journal › Article › peer-review

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abstract = "The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.",

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AU - Tsujimoto, Kazuo

AU - Miyauchi, Seiji

AU - Kamo, Naoki

AU - Ohta, Nobuhiro

PY - 2007/2/5

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AB - The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

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A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

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