A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

Takakazu Nakabayashi; Hui Ping Wang; Kazuo Tsujimoto; Seiji Miyauchi; Naoki Kamo; Nobuhiro Ohta

doi:10.1246/cl.2007.206

A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

Takakazu Nakabayashi, Hui Ping Wang, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

Original language	English
Pages (from-to)	206-207
Number of pages	2
Journal	Chemistry Letters
Volume	36
Issue number	2
DOIs	https://doi.org/10.1246/cl.2007.206
Publication status	Published - 2007 Feb 5
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.2007.206

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abstract = "The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.",

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AU - Nakabayashi, Takakazu

AU - Wang, Hui Ping

AU - Tsujimoto, Kazuo

AU - Miyauchi, Seiji

AU - Kamo, Naoki

AU - Ohta, Nobuhiro

PY - 2007/2/5

Y1 - 2007/2/5

N2 - The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

AB - The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacteriun salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

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DO - 10.1246/cl.2007.206

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ER -

A correlation between pH and fluorescence lifetime of 2′,7′- bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

Abstract

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