A convenient method for preparation of high-purity, Amadori-glycated phosphatidylethanolamine and its prooxidant effect

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6 Citations (Scopus)

Abstract

Phospholipid-linked glycation has been implicated to play an important role in lipid peroxidation associated with several conditions, such as normal aging and diabetes. In this study, we established a convenient method for the preparation of a high-purity, Amadori-glycated phosphatidylethanolamine (Amadori-PE) standard in a methanol system. We evaluated the pro-oxidant effect of the Amadori-PE standard. When Amadori-PE was incubated in a micellar and liposome system in the presence of metal ion, a remarkable increase of lipid peroxidation was observed in both systems. In addition, the Amadori-PE-induced lipid peroxidation was effectively inhibited by superoxide dismutase, mannitol, catalase, and EDTA. These results indicate that Amadori-PE is a considerable risk factor that may cause membrane lipid peroxidation in the pathogenesis of diabetes and aging.

Original languageEnglish
Pages (from-to)276-279
Number of pages4
JournalAnnals of the New York Academy of Sciences
Volume1043
DOIs
Publication statusPublished - 2005 Jan 1

Keywords

  • Amadori product
  • Diabetes mellitus
  • Glycation
  • Lipid peroxidation
  • Phosphatidylethanolamine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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