A comprehensive analysis of the effects of the main component enzymes of cellulase derived from Trichoderma reesei on biomass saccharification

Tetsushi Kawai, Hikaru Nakazawa, Noriko Ida, Hirofumi Okada, Wataru Ogasawara, Yasushi Morikawa, Yoshinori Kobayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aim of this study was a comprehensive analysis of the effects of the component enzymes of cellulase derived from Trichoderma reesei strain PC-3-7 on biomass saccharification. We used cellulases with deleted CBH I, CBH II, or EG I, which contain all other component enzymes, for saccharification of differently pretreated biomasses of rice straw, Erianthus, eucalyptus, and Japanese cedar. We found that CBH I was the most effective in saccharification of all pretreated cellulosic biomasses, although the effect was weaker in saccharification of sulfuric acid- and hydrothermally pretreated rice straw than of others; CBH II was more effective for rice straw than for eucalyptus, and was the most effective at the early stages of biomass degradation; EG I had little effect on pretreated biomasses, in particular, it had no effect on steam-exploded Japanese cedar. Thus, the effects of the main component enzymes depend on the biomass source and pretreatment. These findings will likely help to improve cellulase for industrial use.

Original languageEnglish
Pages (from-to)805-810
Number of pages6
JournalJournal of Industrial Microbiology and Biotechnology
Volume40
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1
Externally publishedYes

Keywords

  • Bioconversion
  • Cellulase
  • Component enzymes
  • Enzymatic saccharification
  • Trichoderma reesei

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'A comprehensive analysis of the effects of the main component enzymes of cellulase derived from Trichoderma reesei on biomass saccharification'. Together they form a unique fingerprint.

  • Cite this