A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals

Hiroshi P.M. Horikawa, Hideo Saisu, Toru Ishizuka, Yoko Sekine, Akira Tsugita, Shoji Odani, Teruo Abe

    Research output: Contribution to journalArticlepeer-review

    76 Citations (Scopus)

    Abstract

    Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

    Original languageEnglish
    Pages (from-to)236-240
    Number of pages5
    JournalFEBS Letters
    Volume330
    Issue number2
    DOIs
    Publication statusPublished - 1993 Sep 13

    Keywords

    • Neurotransmitter release
    • Rab3A/smg25A
    • SNAP-25
    • Syntaxin
    • VAMP/synaptobrevin-2

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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