A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells

Hajime Kurosaki, Yasuhiro Kazuki, Masaharu Hiratsuka, Toshiaki Inoue, Yasuhisa Matsui, Chi Chiu Wang, Mito Kanatsu-Shinohara, Takashi Shinohara, Tosifusa Toda, Mitsuo Oshimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Germline stem (GS) cells can only differentiate into germline cells, while multipotent germ stem (mGS) cells, like embryonic stem (ES) cells, can differentiate into various somatic cells and tissues. The proteomic profiles in GS and mGS cells were compared by two-dimensional gel electrophoresis. Ten down-regulated and 16 up-regulated proteins were differentially expressed in mGS cells in comparison to GS cells, and these proteomic characteristics were very much similar to those in ES cells indicating that multipotency of mGS and ES cells is based on a common molecular event(s). Protein identification by mass spectrometry revealed that these proteins were functionally involved in cell signaling, transcription factors, metabolism, and protein folding. The identified proteins in the present study may thus reveal its biological characteristics and functional property in self-renewal and multipotency.

Original languageEnglish
Pages (from-to)259-267
Number of pages9
JournalBiochemical and biophysical research communications
Volume353
Issue number2
DOIs
Publication statusPublished - 2007 Feb 9

Keywords

  • Embryonal carcinoma cells
  • Embryonic germ cells
  • Embryonic stem cells
  • Germline stem cells
  • Multipotency
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kurosaki, H., Kazuki, Y., Hiratsuka, M., Inoue, T., Matsui, Y., Wang, C. C., Kanatsu-Shinohara, M., Shinohara, T., Toda, T., & Oshimura, M. (2007). A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells. Biochemical and biophysical research communications, 353(2), 259-267. https://doi.org/10.1016/j.bbrc.2006.12.025