Abstract
The effect of geometrical isomers of 9,12-octadecadienoic (18:2) acid on ketogenesis and lipid secretion was compared in isolated perfused rat liver. The hepatic uptake of 18:2 acid isomers was similar in the cis,cis (cc)-, trans,trans (tt)-and a mixture of cis,trans/trans,cis (ct/tc)-isomers. The trans-isomers in comparison with the cis-counterpart stimulated ketogenesis, while reduced hepatic secretion of triacylglycerol and cholesterol and the concentration of triacylglycerol in the post-perfused liver. This reciprocal response was dependent on the number of trans double bonds. The trans-isomers infused during the 4h-perfusion periods were actively incorporated into hepatic lipids and secreted at the expense of endogenous cc-18:2 acid, although the mixture of the mono-trans isomers as compared to the di-trans isomer incorporated more into liver lipids except for phospholipid. On the other hand, less ct-18:2 was incorporated than tc-18:2 into both hepatic and perfusate lipids. These results indicate that both the position and the number of the trans-double bonds in the 18:2 acid determine the pathways of oxidation and esterification.
| Original language | English |
|---|---|
| Pages (from-to) | 779-786 |
| Number of pages | 8 |
| Journal | Nutrition Research |
| Volume | 13 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1993 Jul |
| Externally published | Yes |
Keywords
- Ketone body production
- Perfused rat liver
- Trans-fatty acid
- Triacylglycerol secretion
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Nutrition and Dietetics
