A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer

Osamu Wakao, Ken Satou, Ayano Nakamura, Ken Sumiyoshi, Masanori Shirokawa, Chikaaki Mizokuchi, Kunihiro Shiota, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Fluorescence polarization (FP) offers easy operation and rapid processing, making it implementable in molecular interaction analysis. Previously we have developed a unique FP measurement system using a liquid crystal (LC) layer and an image sensor. The system is based on a principle of synchronized detection between the switching rate of the LC layer and the sampling rate of the CCD. The FP system realized simultaneous multiple sample detection; however, the measurement precision was lower than that of the conventional FP apparatus. The main drawbacks were low light transmittance of the LC layer and insufficient synchronization between the LC layer and CCD. In this paper, we developed a new FP analyzer based on LC-CCD synchronization detection. By using a newly designed LC with high transmittance and improving synchronization, the performance of the system has been dramatically improved. Additionally, we reduced the cost by using an inexpensive CCD and an LED as the excitation source. Simultaneous FP immunoassay of multiple samples of prostaglandin E2 was performed. The error rate of the FP system is reduced from 16.9% to 3.9%, as comparable to the commercial conventional FP system.

Original languageEnglish
Article number024103
JournalReview of Scientific Instruments
Volume89
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

ASJC Scopus subject areas

  • Instrumentation

Fingerprint Dive into the research topics of 'A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer'. Together they form a unique fingerprint.

Cite this