A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Kosuke Fujita; Kumiko Shimomura; Kei ichiro Yamamoto; Tetsuro Yamashita; Koichi Suzuki

doi:10.1016/j.bbrc.2006.04.126

A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Kosuke Fujita, Kumiko Shimomura, Kei ichiro Yamamoto, Tetsuro Yamashita, Koichi Suzuki

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause.

Original language	English
Pages (from-to)	502-507
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	345
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.04.126
Publication status	Published - 2006 Jun 23
Externally published	Yes

Keywords

Chitinase
Feeding behavior
GH48
Glycoside hydrolase
Insect reproductive diapause

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.04.126

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A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle. / Fujita, Kosuke; Shimomura, Kumiko; Yamamoto, Kei ichiro; Yamashita, Tetsuro; Suzuki, Koichi.

In: Biochemical and biophysical research communications, Vol. 345, No. 1, 23.06.2006, p. 502-507.

Research output: Contribution to journal › Article › peer-review

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title = "A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle",

abstract = "Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause.",

keywords = "Chitinase, Feeding behavior, GH48, Glycoside hydrolase, Insect reproductive diapause",

author = "Kosuke Fujita and Kumiko Shimomura and Yamamoto, {Kei ichiro} and Tetsuro Yamashita and Koichi Suzuki",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for JSPS Fellows (No. 16 5403, 2004) and a Grant-in-Aid for the 21st Century Center of Excellence Program (COE) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. ",

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AU - Shimomura, Kumiko

AU - Yamamoto, Kei ichiro

AU - Yamashita, Tetsuro

AU - Suzuki, Koichi

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for JSPS Fellows (No. 16 5403, 2004) and a Grant-in-Aid for the 21st Century Center of Excellence Program (COE) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Abstract

Keywords

ASJC Scopus subject areas

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