A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Kosuke Fujita; Kumiko Shimomura; Kei ichiro Yamamoto; Tetsuro Yamashita; Koichi Suzuki

doi:10.1016/j.bbrc.2006.04.126

A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Kosuke Fujita, Kumiko Shimomura, Kei ichiro Yamamoto, Tetsuro Yamashita, Koichi Suzuki

Research output: Contribution to journal › Article

42 Citations (Scopus)

Abstract

Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause.

Original language	English
Pages (from-to)	502-507
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	345
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.04.126
Publication status	Published - 2006 Jun 23
Externally published	Yes

Keywords

Chitinase
Feeding behavior
GH48
Glycoside hydrolase
Insect reproductive diapause

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Fujita, K., Shimomura, K., Yamamoto, K. I., Yamashita, T., & Suzuki, K. (2006). A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle. Biochemical and biophysical research communications, 345(1), 502-507. https://doi.org/10.1016/j.bbrc.2006.04.126

A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle. / Fujita, Kosuke; Shimomura, Kumiko; Yamamoto, Kei ichiro; Yamashita, Tetsuro; Suzuki, Koichi.

In: Biochemical and biophysical research communications, Vol. 345, No. 1, 23.06.2006, p. 502-507.

Research output: Contribution to journal › Article

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