Abstract
Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause.
Original language | English |
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Pages (from-to) | 502-507 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 345 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2006 Jun 23 |
Externally published | Yes |
Keywords
- Chitinase
- Feeding behavior
- GH48
- Glycoside hydrolase
- Insect reproductive diapause
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology