A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle

Kosuke Fujita, Kumiko Shimomura, Kei ichiro Yamamoto, Tetsuro Yamashita, Koichi Suzuki

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
JournalBiochemical and biophysical research communications
Volume345
Issue number1
DOIs
Publication statusPublished - 2006 Jun 23
Externally publishedYes

Keywords

  • Chitinase
  • Feeding behavior
  • GH48
  • Glycoside hydrolase
  • Insect reproductive diapause

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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