TY - JOUR
T1 - A chicken monoclonal antibody with specificity for the N-terminal of human prion protein
AU - Matsuda, Haruo
AU - Mitsuda, Hiroyuki
AU - Nakamura, Naoto
AU - Furusawa, Shuichi
AU - Mohri, Shirou
AU - Kitamoto, Tetsuyuki
N1 - Funding Information:
We thank Dr. D. Higgins of University of Hong Kong for critical review of the manuscript. This work was partly supported by a Grant-in-Aid for Exploratory Research from the Ministry of Education, Science and Culture, a Grant-in-Aid for Scientific Research from the Ministry of Health and Welfare and a Grant-in-Aid (Research Project for Studies on Pathogenesis of Prion Disease) from the Ministry of Agriculture, Forestry and Fisheries, Japan.
PY - 1999/3
Y1 - 1999/3
N2 - Chickens were immunized with human prion protein (PrP) peptide H25 (amino acid residues 25-49) coupled to keyhole limpet hemocyanin. From a fusion experiment using the chicken fusion partner cell line MuH1 and immune spleen cells, one mAb, HUC2-13, was generated which reacted with the peptide. HUC2-13 was specific for a pentapeptide (RPKPG) of the N-terminal of the peptide H25. In Western blotting analysis, the mAb reacted with PrP materials from a human Creutzfeldt-Jakob disease (CJD) case and the membrane fraction from normal murine brain, but not with the same materials pretreated with proteinase K. When compared with the HUC2-13 and the conventional mouse mAb 3F4, the background stainings using the HUC2-13 were minimal. In immunohistochemistry, the HUC2-13 stained positively with kuru plaques in brain sections from patients with Gerstmann-Straussler syndrome (GSS), and also reacted with synaptic structures of the CJD patients. However, any immunolabelings using the HUC2-13 were not observed in the section from a patient with amyotrophic lateral sclerosis (ALS) as CJD-negative control. These results indicate that the mAb HUC2-13 is a suitable tool for immunological and diagnostic analyses of prion disease in humans and other mammals. Copyright (C) 1999 Federation of European Microbiological Societies.
AB - Chickens were immunized with human prion protein (PrP) peptide H25 (amino acid residues 25-49) coupled to keyhole limpet hemocyanin. From a fusion experiment using the chicken fusion partner cell line MuH1 and immune spleen cells, one mAb, HUC2-13, was generated which reacted with the peptide. HUC2-13 was specific for a pentapeptide (RPKPG) of the N-terminal of the peptide H25. In Western blotting analysis, the mAb reacted with PrP materials from a human Creutzfeldt-Jakob disease (CJD) case and the membrane fraction from normal murine brain, but not with the same materials pretreated with proteinase K. When compared with the HUC2-13 and the conventional mouse mAb 3F4, the background stainings using the HUC2-13 were minimal. In immunohistochemistry, the HUC2-13 stained positively with kuru plaques in brain sections from patients with Gerstmann-Straussler syndrome (GSS), and also reacted with synaptic structures of the CJD patients. However, any immunolabelings using the HUC2-13 were not observed in the section from a patient with amyotrophic lateral sclerosis (ALS) as CJD-negative control. These results indicate that the mAb HUC2-13 is a suitable tool for immunological and diagnostic analyses of prion disease in humans and other mammals. Copyright (C) 1999 Federation of European Microbiological Societies.
KW - Chicken monoclonal antibody
KW - Creutzfeldt-Jakob disease
KW - Gerstmann-Straussler syndrome
KW - Prion protein
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U2 - 10.1016/S0928-8244(98)00135-7
DO - 10.1016/S0928-8244(98)00135-7
M3 - Article
C2 - 10219590
AN - SCOPUS:0033043492
VL - 23
SP - 189
EP - 194
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 3
ER -