A chicken monoclonal antibody with specificity for the N-terminal of human prion protein

Haruo Matsuda, Hiroyuki Mitsuda, Naoto Nakamura, Shuichi Furusawa, Shirou Mohri, Tetsuyuki Kitamoto

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Chickens were immunized with human prion protein (PrP) peptide H25 (amino acid residues 25-49) coupled to keyhole limpet hemocyanin. From a fusion experiment using the chicken fusion partner cell line MuH1 and immune spleen cells, one mAb, HUC2-13, was generated which reacted with the peptide. HUC2-13 was specific for a pentapeptide (RPKPG) of the N-terminal of the peptide H25. In Western blotting analysis, the mAb reacted with PrP materials from a human Creutzfeldt-Jakob disease (CJD) case and the membrane fraction from normal murine brain, but not with the same materials pretreated with proteinase K. When compared with the HUC2-13 and the conventional mouse mAb 3F4, the background stainings using the HUC2-13 were minimal. In immunohistochemistry, the HUC2-13 stained positively with kuru plaques in brain sections from patients with Gerstmann-Straussler syndrome (GSS), and also reacted with synaptic structures of the CJD patients. However, any immunolabelings using the HUC2-13 were not observed in the section from a patient with amyotrophic lateral sclerosis (ALS) as CJD-negative control. These results indicate that the mAb HUC2-13 is a suitable tool for immunological and diagnostic analyses of prion disease in humans and other mammals. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume23
Issue number3
DOIs
Publication statusPublished - 1999 Mar

Keywords

  • Chicken monoclonal antibody
  • Creutzfeldt-Jakob disease
  • Gerstmann-Straussler syndrome
  • Prion protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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