A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator

Seiichi Uchiyama; Toshikazu Tsuji; Kyoko Kawamoto; Kentaro Okano; Eiko Fukatsu; Takahiro Noro; Kumiko Ikado; Sayuri Yamada; Yuka Shibata; Teruyuki Hayashi; Noriko Inada; Masaru Kato; Hideki Koizumi; Hidetoshi Tokuyama

doi:10.1002/anie.201801495

A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator

Seiichi Uchiyama, Toshikazu Tsuji, Kyoko Kawamoto, Kentaro Okano, Eiko Fukatsu, Takahiro Noro, Kumiko Ikado, Sayuri Yamada, Yuka Shibata, Teruyuki Hayashi, Noriko Inada, Masaru Kato, Hideki Koizumi, Hidetoshi Tokuyama

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A cationic fluorescent nanogel thermometer based on thermo-responsive N-isopropylacrylamide and environment-sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10 min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02–0.84 °C in the range 20–40 °C), and remarkable non-cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells.

Original language	English
Pages (from-to)	5413-5417
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	57
Issue number	19
DOIs	https://doi.org/10.1002/anie.201801495
Publication status	Published - 2018 May 4
Externally published	Yes

Keywords

fluorescence spectroscopy
fluorescent probes
imaging agents
nanoparticles
sensors

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201801495

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Uchiyama, S., Tsuji, T., Kawamoto, K., Okano, K., Fukatsu, E., Noro, T., Ikado, K., Yamada, S., Shibata, Y., Hayashi, T., Inada, N., Kato, M., Koizumi, H., & Tokuyama, H. (2018). A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator. Angewandte Chemie - International Edition, 57(19), 5413-5417. https://doi.org/10.1002/anie.201801495

Uchiyama, S, Tsuji, T, Kawamoto, K, Okano, K, Fukatsu, E, Noro, T, Ikado, K, Yamada, S, Shibata, Y, Hayashi, T, Inada, N, Kato, M, Koizumi, H & Tokuyama, H 2018, 'A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator', Angewandte Chemie - International Edition, vol. 57, no. 19, pp. 5413-5417. https://doi.org/10.1002/anie.201801495

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abstract = "A cationic fluorescent nanogel thermometer based on thermo-responsive N-isopropylacrylamide and environment-sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10 min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02–0.84 °C in the range 20–40 °C), and remarkable non-cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells.",

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author = "Seiichi Uchiyama and Toshikazu Tsuji and Kyoko Kawamoto and Kentaro Okano and Eiko Fukatsu and Takahiro Noro and Kumiko Ikado and Sayuri Yamada and Yuka Shibata and Teruyuki Hayashi and Noriko Inada and Masaru Kato and Hideki Koizumi and Hidetoshi Tokuyama",

note = "Funding Information: We are grateful for financial support from the Development of Advanced Measurement and Analysis System by JST, Grant-in-Aid for Scientific Research (B) (17H03075) by JSPS, Grant-in-Aid for Scientific Research (A) (26253001) by JSPS, and Grant-in-Aid Scientific Research on Innovative Areas (16H00999 and 16H01127) by MEXT and JSPS. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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AU - Kawamoto, Kyoko

AU - Okano, Kentaro

AU - Fukatsu, Eiko

AU - Noro, Takahiro

AU - Ikado, Kumiko

AU - Yamada, Sayuri

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AU - Inada, Noriko

AU - Kato, Masaru

AU - Koizumi, Hideki

AU - Tokuyama, Hidetoshi

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A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator

Abstract

Keywords

ASJC Scopus subject areas

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