A cDNA clone encoding an IgE-binding protein from Brassica anther has significant sequence similarity to Ca2+-binding proteins

Kinya Toriyama, Takashi Okada, Masao Watanabe, Takeshi Ide, Tsuneo Ashida, Huiling Xu, Mohan B. Singh

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Thirteen cDNA clones encoding IgE-binding proteins were isolated from expression libraries of anthers of Brassica rapa L. and B. napus L. using serum IgE from a patient who was specifically allergic to Brassica pollen. These clones were divided into two groups, I and II, based on the sequence similarity. All the group I cDNAs predicted the same protein of 79 amino acids, while the group II predicted a protein of 83 amino acids with microheterogeneity. Both of the deduced amino acid sequences contained two regions with sequence similarity to Ca2+-binding sites of Ca2+-binding proteins such as calmodulin. However flanking sequences were distinct from that of calmodulin or other Ca2+-binding proteins. RNA-gel blot analysis showed the genes of group I and II were preferentially expressed in anthers at the later developmental stage and in mature pollen. The recombinant proteins produced in Escherichia coli was recognized in immunoblot analysis by the IgE of a Brassica pollen allergic patient, but not by the IgE of a non-allergic patient. The cDNA clones reported here, therefore, represent pollen allergens of Brassica species.

Original languageEnglish
Pages (from-to)1157-1165
Number of pages9
JournalPlant Molecular Biology
Volume29
Issue number6
DOIs
Publication statusPublished - 1995 Dec 1

Keywords

  • Anther
  • Brassica napus
  • Brassica rapa
  • Ca-binding protein
  • cDNA cloning
  • pollen allergen

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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