A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice

Shinya Fujii, Tomohiko Kazama, Yukihiro Ito, Soichi Kojima, Kinya Toriyama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Findings: To elucidate the protein that interacts with RF2, we performed yeast two-hybrid screening. We identified four genes and named RF2-interacting candidate factors (RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria. A pull-down assay using E. coli-produced recombinant GST-tagged RF2 and His-tagged RIF2 confirmed that RF2 interacted with RIF2. RIF2 encodes ubiquitin domain-containing protein.

Conclusions: These results suggest that RIF2 is a candidate factor of a fertility restoration complex of RF2.

Background: The pollen function of cytoplasmic male sterile (CMS) plants is often recovered by the Restorer of fertility (Rf) gene encoded by the nuclear genome. An Rf gene of Lead rice type CMS, Rf2, encodes a small mitochondrial glycine-rich protein. RF2 is expected to function by interacting with other proteins, because RF2 has no motifs except for glycine-rich domain.

Original languageEnglish
Article number21
JournalRice
Volume7
Issue number1
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

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