TY - JOUR
T1 - A caenorhabditis elegans insulin-like peptide, INS-17
T2 - Its physiological function and expression pattern
AU - Matsunaga, Yohei
AU - Nakajima, Kensuke
AU - Gengyo-Ando, Keiko
AU - Mitani, Shohei
AU - Iwasaki, Takashi
AU - Kawano, Tsuyoshi
N1 - Funding Information:
We thank Dr. Takeshi Ishihara of Kyushu University and Dr. Hiroshi Kagoshima of the Japanese National Institute of Genetics for providing us with the reporter plasmids pPD venus and pHK mRFP respectively. We are also grateful to Dr. James M. Kramer of Northwest- ern University for providing us with marker plasmid pRF4. We thank the Caenorhabditis Genetic Center for providing us with C. elegans strains daf-2(e1370) and daf-16(mu86). This work was supported in part by a grant-in-aid (no. 23580150, to T.K.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - The insulin/insulin-like growth factor-1 signaling pathway of Caenorhabditis elegans regulates larval diapause and adult lifespan through the sole insulin receptor-like protein, DAF-2. In the present study, the physiological function and expression pattern of INS-17, one of the C. elegans insulin-like peptides, were examined by disruption and overexpression of the gene, and by the use of a reporter gene. INS-17 might function as a DAF-2 antagonist in the regulation of larval diapause, but not of the adult lifespan. The reporter protein was intensively expressed during larval diapause. It showed a drastic decrease in amount after larval diapause, which matches well the physiological function of INS-17.
AB - The insulin/insulin-like growth factor-1 signaling pathway of Caenorhabditis elegans regulates larval diapause and adult lifespan through the sole insulin receptor-like protein, DAF-2. In the present study, the physiological function and expression pattern of INS-17, one of the C. elegans insulin-like peptides, were examined by disruption and overexpression of the gene, and by the use of a reporter gene. INS-17 might function as a DAF-2 antagonist in the regulation of larval diapause, but not of the adult lifespan. The reporter protein was intensively expressed during larval diapause. It showed a drastic decrease in amount after larval diapause, which matches well the physiological function of INS-17.
KW - Antagonist
KW - Caenorhabditis elegans
KW - Insulinlike peptide
KW - Larval diapause
KW - Spatio-temporal expression
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U2 - 10.1271/bbb.120540
DO - 10.1271/bbb.120540
M3 - Article
C2 - 23132577
AN - SCOPUS:84870201491
VL - 76
SP - 2168
EP - 2172
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -