A brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits

Feng Li, Hiroyasu Kitashiba, Kiyofumi Inaba, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

For identification of genes responsible for varietal differences in flowering time and leaf morphological traits, we constructed a linkage map of Brassica rapa DNA markers including 170 EST-based markers, 12 SSR markers, and 59 BAC sequence-based markers, of which 151 are single nucleotide polymorphism (SNP) markers. By BLASTN, 223 markers were shown to have homologous regions in Arabidopsis thaliana, and these homologous loci covered nearly the whole genome of A. thaliana. Synteny analysis between B. rapa and A. thaliana revealed 33 large syntenic regions. Three quantitative trait loci (QTLs) for flowering time were detected. BrFLC1 and BrFLC2 were linked to the QTLs for bolting time, budding time, and flowering time. Three SNPs in the promoter, which may be the cause of low expression of BrFLC2 in the early-flowering parental line, were identified. For leaf lobe depth and leaf hairiness, one major QTL corresponding to a syntenic region containing GIBBERELLIN 20 OXIDASE 3 and one major QTL containing BrGL1, respectively, were detected. Analysis of nucleotide sequences and expression of these genes suggested possible involvement of these genes in leaf morphological traits.

Original languageEnglish
Pages (from-to)311-323
Number of pages13
JournalDNA Research
Volume16
Issue number6
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • Bolting time
  • DNA markers
  • Leaf hairiness
  • Leaf lobe
  • Synteny

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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