A BODIPY fluorescent probe with selective response for hypochlorous acid and its application in cell imaging

Lizhi Gai; John MacK; Hui Liu; Zheng Xu; Hua Lu; Zhifang Li

doi:10.1016/j.snb.2013.02.106

A BODIPY fluorescent probe with selective response for hypochlorous acid and its application in cell imaging

Lizhi Gai, John MacK, Hui Liu, Zheng Xu, Hua Lu, Zhifang Li

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

A boron-dipyrromethene (BODIPY) derivative with a hydroxymethyl group at the meso-position, 1, was synthesized, which exhibits a highly specific and rapid 'turn-off' response for HOCl during confocal fluorescence microscopy experiments under physiological conditions and in live cells. The formation of an electron-withdrawing formyl substituent upon oxidation results in highly efficient nonradiative decay of the S₁ state and hence in a drastic decrease in fluorescence emission intensity.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	182
DOIs	https://doi.org/10.1016/j.snb.2013.02.106
Publication status	Published - 2013
Externally published	Yes

Keywords

BODIPY
Cell image
Fluorescent probe
Hypochlorous acid
TD-DFT

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2013.02.106

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title = "A BODIPY fluorescent probe with selective response for hypochlorous acid and its application in cell imaging",

abstract = "A boron-dipyrromethene (BODIPY) derivative with a hydroxymethyl group at the meso-position, 1, was synthesized, which exhibits a highly specific and rapid 'turn-off' response for HOCl during confocal fluorescence microscopy experiments under physiological conditions and in live cells. The formation of an electron-withdrawing formyl substituent upon oxidation results in highly efficient nonradiative decay of the S1 state and hence in a drastic decrease in fluorescence emission intensity.",

keywords = "BODIPY, Cell image, Fluorescent probe, Hypochlorous acid, TD-DFT",

author = "Lizhi Gai and John MacK and Hui Liu and Zheng Xu and Hua Lu and Zhifang Li",

note = "Funding Information: This research was financially supported by NSFC (nos. 21101049 and 20903032 ). ",

year = "2013",

doi = "10.1016/j.snb.2013.02.106",

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T1 - A BODIPY fluorescent probe with selective response for hypochlorous acid and its application in cell imaging

AU - Gai, Lizhi

AU - MacK, John

AU - Liu, Hui

AU - Xu, Zheng

AU - Lu, Hua

AU - Li, Zhifang

N1 - Funding Information: This research was financially supported by NSFC (nos. 21101049 and 20903032 ).

PY - 2013

Y1 - 2013

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AB - A boron-dipyrromethene (BODIPY) derivative with a hydroxymethyl group at the meso-position, 1, was synthesized, which exhibits a highly specific and rapid 'turn-off' response for HOCl during confocal fluorescence microscopy experiments under physiological conditions and in live cells. The formation of an electron-withdrawing formyl substituent upon oxidation results in highly efficient nonradiative decay of the S1 state and hence in a drastic decrease in fluorescence emission intensity.

KW - BODIPY

KW - Cell image

KW - Fluorescent probe

KW - Hypochlorous acid

KW - TD-DFT

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DO - 10.1016/j.snb.2013.02.106

M3 - Article

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

ER -

A BODIPY fluorescent probe with selective response for hypochlorous acid and its application in cell imaging

Abstract

Keywords

ASJC Scopus subject areas

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