A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map

Taku Oshima, Hiroji Aiba, Tomoya Baba, Katsutoshi Fujita, Kouji Hayashi, Atsuko Honjo, Keiichi Ikemoto, Toshifumi Inada, Takeshi Itoh, Miwako Kajihara, Kiyotaka Kanai, Kaoru Kashimoto, Sigenobu Kimura, Masanari Kitagawa, Kozo Makino, Shinji Masuda, Takeyoshi Miki, Kiyoshi Mizobuchi, Hirotada Mori, Kouji MotomuraYoshikazu Nakamura, Hiroko Nashimoto, Yoshitaka Nishio, Noriko Saito, Gen Ichi Sampei, Yasushi Seki, Hideaki Tagami, Keiko Takemoto, Chieko Wada, Yoshihiro Yamamoto, Minoru Yano, Takashi Horiuchi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The 718,122 base pair sequence of the Escherichia coli K-12 genome corresponding to the region from 12.7 to 28.0 minutes on the genetic map is described. This region contains at least 681 potential open reading frames, of which 277 (41%) have been previously identified, 147 (22%) are homologous to other known genes, 139 (20%) are identical or similar to the hypothetical genes registered in databases, and the remaining 118 (17%) do not show a significant similarity to any other gene. In this region, we assigned a cluster of cit genes encoding multienzyme citrate lyase, two clusters of fimbrial genes and a set of lysogenic phage genes encoding integrase, excisionase and repressor in the e14 genetic element. In addition, a new valine tRNA gene, designated valZ, and a family of long directly repeated sequences, LDR-A, -B and -C, were found.

Original languageEnglish
Pages (from-to)137-155
Number of pages19
JournalDNA Research
Volume3
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Keywords

  • Escherichia coli K12
  • Genome sequence
  • ORF analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., ... Horiuchi, T. (1996). A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Research, 3(3), 137-155. https://doi.org/10.1093/dnares/3.3.137