A 570-kb DNA sequence of the escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map

Hiroji Aiba, Tomoya Baba, Kouji Hayashi, Toshifumi Inada, Katumi Isono, Takeshi Itoh, Hiroaki Kasai, Kaoru Kashimoto, Shigenobu Kimura, Madoka Kitakawa, Masanari Kitagawa, Kozo Making, Takeyoshi Miki, Kiyoshi Mizobuchi, Hirotada Mori, Tomoko Mori, Kouji Motomura, Shinsuke Nakade, Yoshikazu Nakamura, Hiroko NashimotoYoshitaka Nishio, Taku Oshima, Noriko Saito, Gen Ichi Sampei, Yasushi Seki, Suharnan Sivasundaram, Hideaki Tagami, Jun Ichi Takeda, Keiko Takemoto, Yasushi Takeuchi, Chieko Wada, Yoshihiro Yamamoto, Takashi Horiuchi

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid β-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.

Original languageEnglish
Pages (from-to)363-377
Number of pages15
JournalDNA Research
Volume3
Issue number6
DOIs
Publication statusPublished - 1996

Keywords

  • Escherichia coli K12
  • Genome sequence
  • ORF analysis
  • Replication termination region

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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