A 460-kb DNA sequence of the escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map

Takeshi Itoh, Hiroji Aiba, Tomoya Baba, Kouji Hayashi, Toshifumi Inada, Katumi Isono, Hiroaki Kasai, Shigenobu Kimura, Madoka Kitakawa, Masanari Kitagawa, Kozo Making, Takeyoshi Miki, Kiyoshi Mizobuchi, Hirotada Mori, Tomoko Mori, Kouji Motomura, Shinsuke Nakade, Yoshikazu Nakamura, Hiroko Nashimoto, Yoshitaka NishioTaku Oshima, Noriko Saito, Gen Ichi Sampei, Yasushi Seki, Suharnan Sivasundaram, Hideaki Tagami, Jun Ichi Takeda, Keiko Takemoto, Chieko Wada, Yoshihiro Yamamoto, Takashi Horiuchi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the crs-element.

Original languageEnglish
Pages (from-to)379-392
Number of pages14
JournalDNA Research
Volume3
Issue number6
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • Escherichia coli K12
  • Genome sequence
  • ORF analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Making, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., ... Horiuchi, T. (1996). A 460-kb DNA sequence of the escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map. DNA Research, 3(6), 379-392. https://doi.org/10.1093/dnares/3.6.379