TY - JOUR
T1 - A 3‐Mb physical map of the chromosome region 8p21.3‐p22, including a 600‐kb region commonly deleted in human hepatocellular carcinoma, colorectal cancer, and non‐small cell lung cancer
AU - Fujiwara, Yoshiyuki
AU - Ohata, Hiroyuki
AU - Emi, Mitsuru
AU - Okui, Keiko
AU - Koyama, Kumiko
AU - Tsuchiya, Eiju
AU - Nakajima, Toshifusa
AU - Monden, Morito
AU - Mori, Takesada
AU - Kurimasa, Akihito
AU - Oshimura, Mitsuo
AU - Nakamura, Yusuke
PY - 1994/5
Y1 - 1994/5
N2 - To isolate a putative tumor suppressor gene(s), we have constructed a physical map and a detailed deletion map of chromosome region 8p21.3‐p22, where loss of heterozygosity (LOH) has been frequently seen in human hepatocellular carcinomas (HCC), colorectal cancers (CRC), and non‐small cell lung cancers (NSCLC). The smallest commonly deleted region at 8p21.3‐p22 in HCC and CRC was between the loci defined by CI8‐245 and CI8‐2644; in NSCLC, a region between CI8‐1051 and CI8‐2644 was commonly deleted. A contiguous physical map of 12 cosmid markers in the 8p21.3‐p22 region was constructed by means of multi‐color fluorescence in situ hybridization (FISH) and pulsed‐field gel electrophoresis (PFGE). On the basis of this physical map, which spans roughly 3.1 Mb, the estimated sizes of the commonly deleted regions were at most 1.2 Mb in HCC and CRC and 0.6 Mb in NSCLC. As four of the 12 physically ordered markers are located within the 0.6 Mb region commonly deleted in all three tumor types, nearly one fourth to one fifth of the target region has already been covered with cosmid inserts. Genes Chrom Cancer 10:7–14 (1994). © 1994 Wiley‐Liss, Inc.
AB - To isolate a putative tumor suppressor gene(s), we have constructed a physical map and a detailed deletion map of chromosome region 8p21.3‐p22, where loss of heterozygosity (LOH) has been frequently seen in human hepatocellular carcinomas (HCC), colorectal cancers (CRC), and non‐small cell lung cancers (NSCLC). The smallest commonly deleted region at 8p21.3‐p22 in HCC and CRC was between the loci defined by CI8‐245 and CI8‐2644; in NSCLC, a region between CI8‐1051 and CI8‐2644 was commonly deleted. A contiguous physical map of 12 cosmid markers in the 8p21.3‐p22 region was constructed by means of multi‐color fluorescence in situ hybridization (FISH) and pulsed‐field gel electrophoresis (PFGE). On the basis of this physical map, which spans roughly 3.1 Mb, the estimated sizes of the commonly deleted regions were at most 1.2 Mb in HCC and CRC and 0.6 Mb in NSCLC. As four of the 12 physically ordered markers are located within the 0.6 Mb region commonly deleted in all three tumor types, nearly one fourth to one fifth of the target region has already been covered with cosmid inserts. Genes Chrom Cancer 10:7–14 (1994). © 1994 Wiley‐Liss, Inc.
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U2 - 10.1002/gcc.2870100103
DO - 10.1002/gcc.2870100103
M3 - Article
C2 - 7519877
AN - SCOPUS:0028354948
VL - 10
SP - 7
EP - 14
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
SN - 1045-2257
IS - 1
ER -