TY - JOUR
T1 - A 1.2-Megabase BAC/PAC contig spanning the 14q13 breakpoint of t(2;14) in a mirror-image polydactyly patient
AU - Matsumoto, Naomichi
AU - Soeda, Eiichi
AU - Ohashi, Hirofumi
AU - Fujimoto, Masahiro
AU - Kato, Rumiko
AU - Tsujita, Takahiro
AU - Tomita, Hiroaki
AU - Kondo, Shinji
AU - Fukushima, Yoshimitsu
AU - Niikawa, Norio
N1 - Funding Information:
We express our gratitude to Ms. Yasuko Nakao and Miho Nagata for their technical assistance. N.N. was supported in part by Grants-in-Aid for Scienti®c Research (08307019), for Priority Areas (08264228), and for the Human Genome Program (07NP0201) from the Ministry of Education, Science and Culture and by a Grant-in-Aid from the Ministry of Health and Welfare, Japan.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997/10/1
Y1 - 1997/10/1
N2 - We previously assigned a 14q13 breakpoint of t(2;14) in a patient with mirror-image polydactyly to a segment between two loci, AFM200ZH4 and D14S306, within a genetic distance of 0.6 cM. In the present study, we constructed a 1.2-Mb high-resolution physical map with a contig composed of 16 bacterial artificial chromosomes (BACs) and 6 P1-derived artificial chromosomes (PACs) at a region around the breakpoint, extending from D14S75 to D14S728 loci. Thirty-four novel sequence-tagged sites (STSs) were also characterized at this region. Of nine ESTs that had been mapped between D14S75 and D14S288, T99065 was confirmed to be in two BAC clones, B102 and B319. This BAC/PAC contig with STSs is useful for further genomic sequencing, for construction of a transcription map, arid for the isolation of the putative gene for mirror-image polydactyly.
AB - We previously assigned a 14q13 breakpoint of t(2;14) in a patient with mirror-image polydactyly to a segment between two loci, AFM200ZH4 and D14S306, within a genetic distance of 0.6 cM. In the present study, we constructed a 1.2-Mb high-resolution physical map with a contig composed of 16 bacterial artificial chromosomes (BACs) and 6 P1-derived artificial chromosomes (PACs) at a region around the breakpoint, extending from D14S75 to D14S728 loci. Thirty-four novel sequence-tagged sites (STSs) were also characterized at this region. Of nine ESTs that had been mapped between D14S75 and D14S288, T99065 was confirmed to be in two BAC clones, B102 and B319. This BAC/PAC contig with STSs is useful for further genomic sequencing, for construction of a transcription map, arid for the isolation of the putative gene for mirror-image polydactyly.
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U2 - 10.1006/geno.1997.4897
DO - 10.1006/geno.1997.4897
M3 - Article
C2 - 9339355
AN - SCOPUS:0031260223
VL - 45
SP - 11
EP - 16
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -