5-Azacytidine enhances transient expression of a transfected gene in cultured mammalian cells

Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

Abstract

The level of transient expression of the Escherichia coli β-galactosidase (β-Gal) gene transfected into three mammalian cell lines was enhanced by culturing the cells with 1-5 μM 5-azacytidine (5-azaC) after the transfection. This enhancement was similarly observed in four different transfection procedures tested. The enhancement of β-Gal expression was primarily due to the increase in the level of its mRNA. Since 5-azaC did not exhibit any obvious effect on the levels of endogenous gene products examined, it is suggested that 5-azaC may preferentially affect the transient expression of an exogenous gene.

Original languageEnglish
Pages (from-to)617-619
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number6
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • 5-azacytidine
  • DNA methylation
  • mammalian cell culture
  • transient expression
  • β-galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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