5-Aminolevulinate synthase expression and hemoglobin synthesis in a human myelogenous leukemia cell line

Tadashi Nagai, Hideo Harigae, Kazumichi Furuyama, Hiroshi Munakata, Norio Hayashi, Kazuyasu Endo, Shigeru Sassa, Masayuki Yamamoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We examined the effect of hemin, TGF-β1 and cytosine arabinoside (Ara-C) on the levels of mRNAs for the erythroid-specific 5-aminolevulinate synthase (ALAS-E) and γ-globin in various human myelogenous leukemia cell lines. Detailed analyses were also made using one of them, YN-1, which was isolated and established in culture from a patient with chronic myelogenous leukemia. Our results demonstrate that γ-globin protein level and the percentage of benzidine-positive cells in the cell line increased markedly (10- to 30-fold) upon treatment with hemin, TGF-β1, or Ara-C. In contrast, γ-globin mRNA was already markedly expressed prior to treatment in 4 out of 9 cell lines examined, including YN-1, and the level increased only marginally after treatment with hemin. ALAS-E mRNA levels were increased in YN-1 cells after treatment with TGF-β1 and Ara-C, while hemin treatment had little effect. These results indicate that heme supply is insufficient in YN-1 cells and suggest that hemin increases hemoglobin synthesis principally at the post-transcriptional level, whereas TGF-β1 and Ara-C stimulate hemoglobin synthesis by activating efficient endogenous heme supply in the cells.

Original languageEnglish
Pages (from-to)487-495
Number of pages9
JournalJournal of biochemistry
Volume121
Issue number3
DOIs
Publication statusPublished - 1997 Jan 1

Keywords

  • 5-Aminolevulinate synthase
  • Globin mRNA
  • Hemin
  • Hemoglobin synthesis
  • Human leukemia cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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