5α-Reductases in human breast carcinoma: Possible modulator of in situ androgenic actions

Takashi Suzuki, Andrew D. Darnel, Jun Ichi Akahira, Naohiro Ariga, Sayaka Ogawa, Chika Kaneko, Junji Takeyama, Takuya Moriya, Hironobu Sasano

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

The expression of 5α-reductase types 1 and 2 was examined in human breast carcinoma using immunohistochemistry and RT-PCR. Immunoreactivity for 5α-reductase isozymes was also correlated with various clinicopathological parameters to examine possible local regulatory mechanisms of sex steroids, including progesterone and androgens, in human breast carcinoma tissues. Immunoreactivity for 5α-reductase type 1 was detected in the cytoplasm and possibly in the nuclear membrane of tumor cells in 35 of 60 invasive ductal carcinomas (58%), and type 2 signal was detected in 9 of these 60 cases (15%). The results from RT-PCR (n = 8) were consistent with those from immunohistochemistry. A significant positive correlation was detected between 5α-reductase type 1 immunoreactivity and androgen and progesterone receptor A or B labeling indexes, and immunoreactivities of 5α-reductase type 2, 17β-hydroxysteroid dehydrogenase type 5, or 3β-hydroxysteroid dehydrogenase, which recognizes both types I and II. An inverse correlation was detected between 5α-reductase type 1 immunoreactivity and tumor size, histological grade, or Ki-67 labeling index. 5α-Reductase type 2 immunoreactivity was significantly correlated with 17β-hydroxysteroid dehydrogenase type 5 immunoreactivity, but not with other parameters. This study suggests that 5α-reductase type 1 is mainly expressed in human breast carcinoma, which may play an important role in the in situ production and actions of the potent androgen, 5α-dihydrotestosterone, including inhibition of cancer cell proliferation, in hormone-dependent human breast carcinoma.

Original languageEnglish
Pages (from-to)2250-2257
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume86
Issue number5
DOIs
Publication statusPublished - 2001 Jan 1

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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