47-mG2a: A Mouse IgG2a-Type of PcMab-47 Useful for Detecting Podocalyxin in Esophageal Cancers by Immunohistochemistry

Mika K. Kaneko, Shunsuke Itai, Shinji Yamada, Yukinari Kato

Research output: Contribution to journalArticle

Abstract

Esophageal cancer is one of the highly malignant cancers. It comprises two of the most common histological tumor types: squamous cell carcinoma (SCC) and adenocarcinoma. SCC accounts for about 90% of esophageal cancers. Despite developments in treatment strategies, the prognosis and survival rate remain poor. Podocalyxin (PODXL) is a highly glycosylated type-I transmembrane protein. It is expressed in normal tissues such as kidney, heart, breast, and pancreas. Upregulation of PODXL correlates with tumor progression, invasion, and metastasis. Therefore, this glycoprotein could be a potential biomarker for predicting the prognosis of some cancers, for instance, brain, colorectal, oral, lung, bladder, prostate, and ovarian cancers. We previously developed a specific and sensitive anti-PODXL monoclonal antibody (mAb), PcMab-47 (mouse IgG1, kappa) and its mouse IgG2a-type (47-mG2a). We showed their utility in immunohistochemical analysis of oral cancers. Herein, we demonstrate that PcMab-47 and 47-mG2a can also be used to detect esophageal squamous cell carcinoma (ESCC) with this technique. These two antibodies, respectively, stained 123/130 (94.6%) and 127/130 (97.7%) ESCC cases, indicating that they can detect PODXL with high sensitivity in this carcinoma. Of more than 3+ cases, 47-mG2a was more effective than PcMab-47, respectively, staining 56/127 (44.1%) and 41/123 (33.3%). Therefore, 47-mG2a can be used for the detection of PODXL in ESCC using immunohistochemical analysis.

Original languageEnglish
Pages (from-to)158-161
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume37
Issue number3
DOIs
Publication statusPublished - 2018 Jun

Keywords

  • PODXL
  • esophageal cancer
  • monoclonal antibody
  • podocalyxin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of '47-mG<sub>2a</sub>: A Mouse IgG<sub>2a</sub>-Type of PcMab-47 Useful for Detecting Podocalyxin in Esophageal Cancers by Immunohistochemistry'. Together they form a unique fingerprint.

  • Cite this