4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

Atsushi Kobayashi, Tomonari Tanaka, Kazuhito Watanabe, Masaki Ishihara, Masato Noguchi, Hirofumi Okada, Yasushi Morikawa, Shin Ichiro Shoda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-β-XXXG and DMT-β-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-β-1,4-d-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.

Original languageEnglish
Pages (from-to)3588-3591
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number12
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • DMT-MM
  • Glycosidase
  • Oligosaccharide
  • Substrate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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