4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

Atsushi Kobayashi; Tomonari Tanaka; Kazuhito Watanabe; Masaki Ishihara; Masato Noguchi; Hirofumi Okada; Yasushi Morikawa; Shin Ichiro Shoda

doi:10.1016/j.bmcl.2010.04.122

4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

Atsushi Kobayashi, Tomonari Tanaka, Kazuhito Watanabe, Masaki Ishihara, Masato Noguchi, Hirofumi Okada, Yasushi Morikawa, Shin Ichiro Shoda

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-β-XXXG and DMT-β-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-β-1,4-d-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.

Original language	English
Pages (from-to)	3588-3591
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	20
Issue number	12
DOIs	https://doi.org/10.1016/j.bmcl.2010.04.122
Publication status	Published - 2010

Keywords

DMT-MM
Glycosidase
Oligosaccharide
Substrate

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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Kobayashi, A., Tanaka, T., Watanabe, K., Ishihara, M., Noguchi, M., Okada, H., Morikawa, Y., & Shoda, S. I. (2010). 4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei. Bioorganic and Medicinal Chemistry Letters, 20(12), 3588-3591. https://doi.org/10.1016/j.bmcl.2010.04.122

4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans : Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei. / Kobayashi, Atsushi; Tanaka, Tomonari; Watanabe, Kazuhito et al.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 20, No. 12, 2010, p. 3588-3591.

Research output: Contribution to journal › Article › peer-review

Kobayashi, A, Tanaka, T, Watanabe, K, Ishihara, M, Noguchi, M, Okada, H, Morikawa, Y & Shoda, SI 2010, '4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei', Bioorganic and Medicinal Chemistry Letters, vol. 20, no. 12, pp. 3588-3591. https://doi.org/10.1016/j.bmcl.2010.04.122

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title = "4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei",

abstract = "Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-β-XXXG and DMT-β-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-β-1,4-d-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.",

keywords = "DMT-MM, Glycosidase, Oligosaccharide, Substrate",

author = "Atsushi Kobayashi and Tomonari Tanaka and Kazuhito Watanabe and Masaki Ishihara and Masato Noguchi and Hirofumi Okada and Yasushi Morikawa and Shoda, {Shin Ichiro}",

note = "Funding Information: This work was supported by a Grant-in Aid for Scientific Research from the Ministry of Education, Sports, Science and Technology , and Industrial Technology Research Grant Program in 2006 from NEDO of Japan.",

year = "2010",

doi = "10.1016/j.bmcl.2010.04.122",

language = "English",

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pages = "3588--3591",

journal = "Bioorganic and Medicinal Chemistry Letters",

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T2 - Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

AU - Kobayashi, Atsushi

AU - Tanaka, Tomonari

AU - Watanabe, Kazuhito

AU - Ishihara, Masaki

AU - Noguchi, Masato

AU - Okada, Hirofumi

AU - Morikawa, Yasushi

AU - Shoda, Shin Ichiro

N1 - Funding Information: This work was supported by a Grant-in Aid for Scientific Research from the Ministry of Education, Sports, Science and Technology , and Industrial Technology Research Grant Program in 2006 from NEDO of Japan.

PY - 2010

Y1 - 2010

N2 - Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-β-XXXG and DMT-β-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-β-1,4-d-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.

AB - Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-β-XXXG and DMT-β-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-β-1,4-d-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.

KW - DMT-MM

KW - Glycosidase

KW - Oligosaccharide

KW - Substrate

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ER -

4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

Abstract

Keywords

ASJC Scopus subject areas

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