In this paper, we propose 3D space which is based on concept of real and digital spaces symbiosis. Recently, collaborative work environments which are using Augmented Reality have been expected to realize effective collaborative works. However, these environments have some limitations. One of limitations is restricted availability of devices, and another limitation is low usability. Because of these limitations, it is difficult to utilize these environments, for general users. To break these limitations, we propose the construction scheme of 3D symbiotic space. Collaborative work environments based on 3D symbiotic space enable users to do collaborative works intuitively as if virtual spaces united with the real space. First, we present fundamental technologies for 3D symbiotic space. Next, we show the design and implementation of the agent-aided collaborative work environments based on 3D symbiotic space. Finally, we confirm feasibility of 3D symbiotic space with experimental results.