3D electrochemical and ion current imaging using scanning electrochemical-scanning ion conductance microscopy

Yasufumi Takahashi, Hiroki Ida, Yoshiharu Matsumae, Hirokazu Komaki, Yuanshu Zhou, Akichika Kumatani, Makoto Kanzaki, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Local cell-membrane permeability and ionic strength are important factors for maintaining the functions of cells. Here, we measured the spatial electrochemical and ion concentration profile near the sample surface with nanoscale resolution using scanning electrochemical microscopy (SECM) combined with scanning ion-conductance microscopy (SICM). The ion current feedback system is an effective way to control probe-sample distance without contact and monitor the kinetic effect of mediator regeneration and the chemical concentration profile. For demonstrating 3D electrochemical and ion concentration mapping, we evaluated the reaction rate of electrochemical mediator regeneration on an unbiased conductor and visualized inhomogeneous permeability and the ion concentration 3D profile on a single fixed adipocyte cell surface.

Original languageEnglish
Pages (from-to)26728-26733
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume19
Issue number39
DOIs
Publication statusPublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of '3D electrochemical and ion current imaging using scanning electrochemical-scanning ion conductance microscopy'. Together they form a unique fingerprint.

Cite this