3-Phosphoglycerate dehydrogenase in Mesorhizobioum loti is essential for maintaining symbiotic nitrogen fixation of Lotus japonicus root nodules

Nanthipak Thapanapongworakul, Mika Nomura, Tan van Dao, Yoshikazu Shimoda, Shusei Sato, Satoshi Tabata, Shigeyuki Tajima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mesorhizobium loti is a Gram negative bacterium that induces N2-fixing root nodules on the model legume Lotus japonicus. Proteomic analysis in M. loti indicated that 3-phosphoglycerate dehydrogenase (EC. 1.1.1.95, PHGDH) protein content was 2.2 times higher in bacteroids than in cultured bacteria. A M. loti mutant (STM5) with a transposon insertion in the PHGDH gene, mll3875, showed an absolute dependence on serine or glycine in minimal medium for growth. When L. japonicus plants were infected with STM5, the roots formed nodules in numbers comparable to those formed by wild type M. loti; however, the nodules showed very low acetylene reduction activity, and significant starch granule accumulation was observed in the uninfected cells. In such nodules, vast necrosis occurred in the central tissue of the nodules, although bacteroids were detected in the infected cell of the nodules. These data indicate that serine or glycine biosynthesis by PHGDH is important for maintaining symbiosis and nitrogen fixation in L. japonicus nodules.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalPlant and Soil
Volume336
Issue number1
DOIs
Publication statusPublished - 2010 Nov 1
Externally publishedYes

Keywords

  • 3-Phosphoglycerate dehydrogenase
  • Auxotrophs
  • Lotus japonicus
  • Mesorhizobium loti
  • Signature-tagged mutagenesis

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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