2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima

Gohei Hayashi, Carlo F. Moro, Jai Singh Rohila, Junko Shibato, Akihiro Kubo, Tetsuji Imanaka, Shinzo Kimura, Shoji Ozawa, Satoshi Fukutani, Satoru Endo, Katsuki Ichikawa, Ganesh Kumar Agrawal, Seiji Shioda, Motohide Hori, Manabu Fukumoto, Randeep Rakwal

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The present study continues our previous research on investigating the biological effects of low-level gamma radiation in rice at the heavily contaminated Iitate village in Fukushima, by extending the experiments to unraveling the leaf proteome. 14-days-old plants of Japonica rice (Oryza sativa L. cv. Nipponbare) were subjected to gamma radiation level of upto 4 μSv/h, for 72 h. Following exposure, leaf samples were taken from the around 190 μSv/3 d exposed seedling and total proteins were extracted. The gamma irradiated leaf and control leaf (harvested at the start of the experiment) protein lysates were used in a 2-D differential gel electrophoresis (2D-DIGE) experiment using CyDye labeling in order to asses which spots were differentially represented, a novelty of the study. 2D-DIGE analysis revealed 91 spots with significantly different expression between samples (60 positive, 31 negative). MALDI-TOF and TOF/TOF mass spectrometry analyses revealed those as comprising of 59 different proteins (50 up-accumulated, 9 downaccumulated). The identified proteins were subdivided into 10 categories, according to their biological function, which indicated that the majority of the differentially expressed proteins consisted of the general (non-energy) metabolism and stress response categories. Proteome-wide data point to some effects of low-level gamma radiation exposure on the metabolism of rice leaves.

Original languageEnglish
Article numbere1103406
JournalPlant Signaling and Behavior
Volume10
Issue number12
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • 2D-DIGE
  • Fukushima
  • Proteomics
  • Radioactively contaminated soil
  • Rice seedling

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of '2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima'. Together they form a unique fingerprint.

  • Cite this

    Hayashi, G., Moro, C. F., Rohila, J. S., Shibato, J., Kubo, A., Imanaka, T., Kimura, S., Ozawa, S., Fukutani, S., Endo, S., Ichikawa, K., Agrawal, G. K., Shioda, S., Hori, M., Fukumoto, M., & Rakwal, R. (2015). 2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima. Plant Signaling and Behavior, 10(12), [e1103406]. https://doi.org/10.1080/15592324.2015.1103406