2,2′-dihydroxyazobenzene derivatives as reagents for trace metal determination by ion-pair reversed-phase high-performance liquid chromatography with spectrophotometric detection

Hitoshi Hoshino, Kouji Nakanot, Takao Yotsuyanagi

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

2,2′-Dihydroxyazobenzene and five substituted analogues have been examined as pre-column chelating agents for the determination of trace metal ions by reversed-phase high-performance liquid chromatography (RP-HPLC) with spectrophotometric detection. Seven metal ions, viz., AlIII, CoIII, CuIII, FeIII, MoVI, VV and ZnII, were detected as their azo chelates in the ion-pair RP mode. The optimum conditions, including pH for colour formation, column used, mobile phase parameters and detection wavelength, were investigated for each individual reagent. The method was applied to the determination of AI and Fe in coal fly ash samples. The kinetic nature and the forms of the chelates responsible for the selectivity of RP-HPLC towards metal ions are discussed in terms of "kinetic differentiation" together with ion-pair RP partition phenomena.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalThe Analyst
Volume115
Issue number2
DOIs
Publication statusPublished - 1990 Jan 1

Keywords

  • 2,2′-Dihydroxyazobenzene reagent
  • Ion-pair reversed-phase high-performance liquid chromatography
  • Kinetic differentiation
  • Metal chelate
  • Spectrophotometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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