[21] Geranylgeranylpyrophosphate Synthetase of Pig Liver

Hiroshi Sagami; Koichi Ishii; Kyozo Ogura

doi:10.1016/S0076-6879(85)10074-1

[21] Geranylgeranylpyrophosphate Synthetase of Pig Liver

Hiroshi Sagami, Koichi Ishii, Kyozo Ogura

Institute of Multidisciplinary Research for Advanced Materials (IMRAM)

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This chapter describes preparation of geranylgeranylpyrophosphate synthetase from pig liver. Geranylgeranylpyrophosphate synthetase obtained from pig liver in a form free of farnesylpyrophosphate synthetase catalyzes the two reactions, and does not catalyze the condensation between dimethylallyl pyrophosphate and isopentenyl pyrophosphate. Assay methods and purification of geranylgeranylpyrophosphate synthetase are described in the chapter. The enzyme solution obtained by hydroxylapatite chromatography is concentrated by ultrafiltration, and chromatographed through a Sephadex G-200 column (3.0 × 80 cm) with 0.01 M phosphate buffer, pH 6.8. This chapter summarizes the results of purification up to hydroxylapatite chromatography starting with 200 g of pig liver.

Original language	English
Pages (from-to)	184-188
Number of pages	5
Journal	Methods in enzymology
Volume	110
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(85)10074-1
Publication status	Published - 1985 Jan 1

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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abstract = "This chapter describes preparation of geranylgeranylpyrophosphate synthetase from pig liver. Geranylgeranylpyrophosphate synthetase obtained from pig liver in a form free of farnesylpyrophosphate synthetase catalyzes the two reactions, and does not catalyze the condensation between dimethylallyl pyrophosphate and isopentenyl pyrophosphate. Assay methods and purification of geranylgeranylpyrophosphate synthetase are described in the chapter. The enzyme solution obtained by hydroxylapatite chromatography is concentrated by ultrafiltration, and chromatographed through a Sephadex G-200 column (3.0 × 80 cm) with 0.01 M phosphate buffer, pH 6.8. This chapter summarizes the results of purification up to hydroxylapatite chromatography starting with 200 g of pig liver.",

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