2-Methyl-2,4-pentanediol induces spontaneous assembly of staphylococcal α-hemolysin into heptameric pore structure

Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Yoshiyuki Kamio, Min Yao, Isao Tanaka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Staphylococcal α-hemolysin is expressed as a water-soluble monomeric protein and assembles on membranes to form a heptameric pore structure. The heptameric pore structure of α-hemolysin can be prepared from monomer in vitro only in the presence of deoxycholate detergent micelles, artificially constructed phospholipid bilayers, or erythrocytes. Here, we succeeded in preparing crystals of the heptameric form of α-hemolysin without any detergent but with 2-methyl-2,4-pentanediol (MPD), and determined its structure. The structure of the heptameric pore was similar to that reported previously. In the structure, two molecules of MPD were bound around Trp179, around which phospholipid head groups were bound in the heptameric pore structure reported previously. Size exclusion chromatography showed that α-hemolysin did not assemble spontaneously even when stored for 1 year. SDS-PAGE analysis revealed that, among the compounds in the crystallizing buffer, MPD could induce heptamer formation. The concentration of MPD that most efficiently induced oligomerization was between 10 and 30%. Based on these observations, we propose MPD as a reagent that can facilitate heptameric pore formation of α-hemolysin without membrane binding. Published by Wiley-Blackwell.

Original languageEnglish
Pages (from-to)448-456
Number of pages9
JournalProtein Science
Volume20
Issue number2
DOIs
Publication statusPublished - 2011 Feb

Keywords

  • 2-Methyl-2,4-pentanediol (MPD)
  • Crystal structure
  • Heptamer formation
  • Staphylococcal α-hemolysin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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