12-Oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis

Nozomi Taki, Yuko Sasaki-Sekimoto, Takeshi Obayashi, Akihiro Kikuta, Koichi Kobayashi, Takayuki Ainai, Kaori Yagi, Nozomu Sakurai, Hideyuki Suzuki, Tatsuru Masuda, Ken Ichiro Takamiya, Daisuke Shibata, Yuichi Kobayashi, Hiroyuki Ohta

Research output: Contribution to journalArticlepeer-review

329 Citations (Scopus)

Abstract

Jasmonic acid (JA) and methyl jasmonate (MeJA), collectively known as JAs, regulate diverse physiological processes in plants, including the response to wounding. Recent reports suggest that a cyclopentenone precursor of JA, 12-oxo-phytodienoic acid (OPDA), can also induce gene expression. However, little is known about the physiological significance of OPDA-dependent gene expression. We used microarray analysis of approximately 21,500 Arabidopsis (Arabidopsis thaliana) genes to compare responses to JA, MeJA, and OPDA treatment. Although many genes responded identically to both OPDA and JAs, we identified a set of genes (OPDA-specific response genes [ORGs]) that specifically responded to OPDA but not to JAs. ORGs primarily encoded signaling components, transcription factors, and stress response-related genes. One-half of the ORGs were induced by wounding. Analysis using mutants deficient in the biosynthesis of JAs revealed that OPDA functions as a signaling molecule in the wounding response. Unlike signaling via JAs, OPDA signaling was CORONATINE INSENSITIVE 1 independent. These results indicate that an OPDA signaling pathway functions independently of JA/MeJA signaling and is required for the wounding response in Arabidopsis.

Original languageEnglish
Pages (from-to)1268-1283
Number of pages16
JournalPlant physiology
Volume139
Issue number3
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of '12-Oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis'. Together they form a unique fingerprint.

Cite this