β-Phorbol ester-induced enhancement of exocytosis in large mossy fiber boutons of mouse hippocampus

Takuya Hikima, Rikita Araki, Toru Ishizuka, Hiromu Yawo

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    1 Citation (Scopus)

    Abstract

    β-Phorbol esters (BPE), synthetic analogues of diacylglycerol (DAG), induce the potentiation of transmission in many kinds of synapses through activating the C1 domain-containing receptors. However, their effects on synaptic vesicle exocytosis have not yet been investigated. Here, we evaluated the vesicular exocytosis directly from individual large mossy fiber boutons (LMFBs) in hippocampal slices from transgenic mice that selectively express synaptopHluorin (SpH). We found that the activity-dependent increment of SpH fluorescence (ΔSpH) was enhanced by 4β-phorbol 12,13-diacetate (PDAc), one of the BPEs, without influencing the recycled component of SpH. These PDAc effects on ΔSpH were almost completely inhibited by staurosporine, a non-selective antagonist of protein kinases. However, intermittent synaptic transmission was still potentiated through a staurosporine-resistant mechanism. The staurosporine-sensitive cascade may facilitate the vesicle replenishment, thus maintaining the fidelity of transmission at a high level during repetitive firing of the presynaptic neuron.

    Original languageEnglish
    Pages (from-to)263-274
    Number of pages12
    JournalJournal of Physiological Sciences
    Volume59
    Issue number4
    DOIs
    Publication statusPublished - 2009 Jul 1

    Keywords

    • Exocytosis
    • Munc13-1
    • PKC
    • Presynaptic mechanism
    • Synaptic plasticity
    • Synaptic transmission

    ASJC Scopus subject areas

    • Physiology

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