β-Lactamase activity in sputum. (I). Correlation between β-Lactamase activity of sputum and of isolates from sputum

Junichi Chiba, Miwa Kato, Akira Watanabe, Kotaro Oizumi, Masakichi Motomiya

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    6 Citations (Scopus)

    Abstract

    We investigated the correlation between β-lactamase activity determined by the nitrocefin-method for clinical pathogens isolated from sputum and that of sputum per se, and discuss the possible indirect pathogenecity of non-causative organisms. Forty-six microbes, including 10 strains of Pseudomonas aeruginosa, 6 each of Staphylococcus aureus, Serratia marcescens and Enterobacter cloacae, 4 of Xanthomonas maltophilia, 3 of Acinetobacter calcoaceticus, 2 of Haemophilus influenzae and one strain each of Enterococcus faecalis, Branhamella catarrhalis, Klebsiella pneumoniae, Proteus mirabilis, Hafnia alvei, Providencia stuartii, Pseudomonas sp., Flavobacterium meningosepticum and Flavobacterium indologenes were recovered from the sputum of 39 patients with respiratory infections. The β-lactamase activity was negative in 9 of 46 strains. Of the remainder, 13 strains responded slowly and 24 rapidly in their production of β-lactamase.β-Lactamase-positive strains were recovered from 35 of 39 patients. The β-lactamase activity of sputum from 35 cases whose isolates were positive for β-lactamase, was negative in 12, weakly positive in 17 and strongly positive in 6 cases. The β-lactamase activity of sputum from cases whose isolates were negative for β-lactamase, was negative in all four cases. Both β-lactamase producing and non-producing strains were recovered from the same sputum in five of six cases in whom multi-organisms were recovered. The β-lactamase activity of sputum from cases with prior chemotherapy was stroger than that without prior chemotherapy, and the difference was statistically significant (P<0.025). We suggest from the above results that non-causative organisms which are positive for β-lactamase enhance the pathogenecity of causative organisms which are negative for β-lactamase since β-lactamase is produced by non-causative organisms in the sputum.

    Original languageEnglish
    Pages (from-to)1031-1039
    Number of pages9
    JournalChemotherapy
    Volume37
    Issue number8
    DOIs
    Publication statusPublished - 1989 Jan 1

    ASJC Scopus subject areas

    • Pharmacology (medical)
    • Infectious Diseases
    • Pharmacology
    • Drug Discovery
    • Oncology

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