β-Galactosidase, phospho-β-galactosidase and phospho-β- glucosidase activities in lactobacilli strains isolated from human faeces

H. Honda, F. Kataoka, S. Nagaoka, Y. Kawai, H. Kitazawa, H. Itoh, K. Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, T. Saito

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Aims: Lactose intolerance, a serious health problem for Asians, can be solved using probiotic bacteria having high lactose hydrolysis activities. We determined the distribution of β-galactosidase (β-gal), phospho-β-galactosidase (P-βgal) and phospho-β-glucosidase (P-β-glc) activities in species of lactic acid bacteria (LAB) isolated from human faeces to select strains for potential use in fermented dairy products, e.g. yogurt. Methods and Results: The sugar substrates, o-nitrophenyl-β-d- galactopyranoside 6-phosphate and o-nitrophenyl-β-d-glucopyranoside 6-phosphate, were synthesized and used to measure respectively P-β-gal and P-β-glc activities. Sixty-five toluene-treated strains were examined for three lactase enzyme activities. Lactobacillus mucosae OLL2848 showed the highest β-gal activity (107.09 U mg-1 of protein) among the Lactobacillus strains from human faeces. Lactobacillus gasseri OLL2836 and OLL 2948 showed the highest P-β-gal (46.58 U) and P-β-glc (50.19 U)activity, respectively, with no β-gal activity. Conclusions: The expression of P-β-glc induced by lactose was characteristic of Lact. gasseri. Because this LAB is a major inhabitant of the human intestine. This enzyme is a key glycosidase involved in lactose utilization. Significance and Impact of Study: This is the first report describing the distribution of three glycosidase activities used in lactose metabolism in LAB isolated from human faeces for possible use in functional foods.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalLetters in Applied Microbiology
Volume45
Issue number5
DOIs
Publication statusPublished - 2007 Nov

Keywords

  • Lactic acid bacteria
  • Lactobacillus gasseri
  • Lactose
  • Phospho-β- galactosidase
  • Phospho-β-glucosidase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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