α-Tocopherol attenuates the cytotoxic effect of δ-tocotrienol in human colorectal adenocarcinoma cells

Akira Shibata, Kiyotaka Nakagawa, Phumon Sookwong, Tsuyoshi Tsuduki, Akira Asai, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Recent studies have demonstrated that tocotrienol (T3) is superior to tocopherol (Toc) for cancer chemoprevention. However, there is little information on whether Toc influences the anticancer property of T3. In this study, we investigated the influence of Toc on the cytotoxic effects of δ-T3 in DLD-1 human colorectal adenocarcinoma cells. Toc, especially α-Toc, attenuated δ-T3-induced cytotoxicity and apoptosis in DLD-1 cells, whereas Toc alone did not exhibit any cytotoxic effect. δ-T3-induced cell cycle arrest and proapoptotic gene/protein expression (e.g., p21, p27, and caspases) were abrogated by a-Toc. Furthermore, coadministration of α-Toc decreased δ-T3 uptake into DLD-1 cells in a dose-dependent manner. These results indicate that α-Toc is not only less cytotoxic to cancer cells, but it also reduces the cytotoxicity of δ-T3 by inhibiting its cellular uptake.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalBiochemical and biophysical research communications
Volume397
Issue number2
DOIs
Publication statusPublished - 2010 Jun 25

Keywords

  • Apoptosis
  • Cytotoxicity
  • Tocopherol
  • Tocotrienol

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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